QuantiQuik™ Histamine Quick Test Strips

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ProtocolSDS

Application

Direct determination of Histamine concentrations in fish and red wine samples.

Key Features

Fast and sensitive. Use of 500 mg of fish or 250 µL of sample. Semi-quantitative measurement between 0-200 ppm (undiluted) Histamine.
Convenient. No expensive lab equipment needed.
Sample treatment and assay can be performed in under 15 minutes.

Method

Visual

Samples

Seafood, wine, milk, etc

Species

Size

10 tests

Shelf Life

6 months

More Details

HISTAMINE(C₅H₉N₃) is a biogenic amine naturally present in many foods and body cells in amounts without toxicological significance. It is also a contaminant mostly found in Scombridae family fishes, such as tuna and mackerel, as well as other seafood products when they are improperly handled or stored. The consumption of foods containing high levels of histamine may lead to an allergy-like food poisoning known as scombroid poisoning.BioAssay Systems QuantiQuik™ Histamine Quick Test Strips are based on histamine dehydrogenase catalyzed oxidation of histamine in which the formed electron mediator reduces a formazan (MTT) reagent. The intensity of product color is directly proportional to histamine concentration in the sample.

What samples have you tested?

Fish samples: Ahi tuna, Mahi Mahi, Albacore, Mackerel, Anchovy, Salmon, Tilapia. Red wine, white wine, and milk.

How sensitive are the Histamine strips?

The lower detection limit of the strips for seafood, red wine, and milk samples is 20 ppm.

My sample turns the strip very dark purple, how can I determine the Histamine concentration?

Very dark purple indicates that the diluted sample concentration is greater than 200 ppm. To obtain a more accurate concentration, the sample should be diluted and retested. Multiply the concentration of the corresponding color by the dilution factor to determine the histamine concentration.

I don’t have access to a homogenizer. How can I effectively homogenize my fish samples?

Fish samples can be mashed with a tooth pick and then vigorously shaken for 2 minutes in the extraction buffer.

How can I measure levels of histamine in milk samples?

Milk samples require a 2× Dilution. Carefully add 200 µL of Extraction Buffer to a Sample tube. Then, add 200 µL of milk. Replace the cap, securely close the vial, and invert the vial a couple of times to mix the diluted sample. Proceed with step 1 of the test procedure on the product protocol. Dilution Factor=2

Can I measure histamine in white wine samples?

Histamine can be measured in white wine samples, but please note that only levels greater than 20 ppm can be detected and white wine rarely has levels of histamine this high. White wine samples should be diluted 2× in Extraction buffer. See question 5 for instructions.

Can I store unused reagents for future use?

Yes, unused reagents can be stored according to the assay protocol. The strips should be kept in a dry, preferably cool location protected from light. For long term storage (>30 days), we recommend keeping the Extraction Buffer at 4°C or below.For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.

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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.Simply send us your samples:- Fast turnaround - Quality data - Low costPlease email or call 1-510-782-9988 x 2 to request assay service.

品牌介绍

BioAssay Systems开发和销售创新的高通量分析解决方案，以满足生命科学行业不断增长的需求。BioAssay Systems的创始人在现代药物发现领域拥有50多年的丰富经验。在过去的15年中，我们已经开发了200多种用于药物发现和生命科学研究的检测试剂盒。我们以提供的服务和简单，易用且性能卓越的产品而感到自豪。

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