QuantiChrom™ Magnesium Assay Kit

What are typical optical density readings of deionized water and the magnesium standard in this assay?

The OD reading will vary with the experimental conditions and the OD reader used. In our laboratory, the OD(500 nm) for distilled water is typically around 0.300, and after the addition of EDTA basically unchanged, for instance 0.296. For the magnesium standard, the values are typically around 0.450 and 0.295, respectively.

What is the variability across replicated well samples?

The intra-assay variability is typically below 5%.

Do you recommend me to dilute my samples for the Magnesium Assay Kit?

Whether you need to dilute your sample depends on the nature of the sample and the expected concentration of Mg. Normal Mg levels in blood are 1.7-2.3 mg/dL and fall within the linear detection range of 0.1 to 3 mg/dL - dilution is thus not necessary. On the other hand for urine samples, normal values are typically higher, around 7.2 -10.8 mg/dL, and a dilution of the sample is necessary.For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.

Liu, M., Jeong, E. M., Liu, H., Xie, A., So, E. Y., Shi, G. & Dudley Jr, S. C. (2019). Magnesium supplementation improves diabetic mitochondrial and cardiac diastolic function. JCI insight, 4(1). Assay: Magnesium in mice blood. Ando, I., Karasawa, K., Yokota, S., Shioya, T., Matsuda, H., & Tanaka, A. (2017). Analysis of serum magnesium ions in dogs exposed to external stress: A pilot study. Open veterinary journal, 7(4), 367-374. Assay: Magnesium in dog serum. Kingman, J., Uitto, J., & Li, Q. (2017). Elevated dietary magnesium during pregnancy and postnatal life prevents ectopic mineralization in Enpp1asj mice, a model for generalized arterial calcification of infancy. Oncotarget, 8(24), 38152. Assay: Magnesium in mice serum. Ballester-Lozano GF et al. (2015). Comprehensive biometric, biochemical and histopathological assessment of nutrient deficiencies in gilthead sea bream fed semi-purified diets. Br J Nutr. 114(5):713-26. Assay: magnesium in fish plasma. Breiderhoff, T et al (2012). Deletion of claudin-10 (Cldn10) in the thick ascending limb impairs paracellular sodium permeability and leads to hypermagnesemia and nephrocalcinosis. Proceedings of the National Academy of Sciences 109(35): 14241-14246. Assay: Magnesium in mouse urine, serum. Ullah, S., Khan, A. A., Khan, M. A., & Ahmed, I. (2012). Effects of gasoline engine emissions on health status of auto rickshaw drivers at DIR (L) Khyber Pukhtunkhwa. Toxicological & Environmental Chemistry 94(2): 411-422. Assay: Magnesium in human blood. Elizondo MR, et al (2010). trpm7 regulation of in vivo cation homeostasis and kidney function involves stanniocalcin 1 and fgf23. Endocrinology 151(12):5700-9. Assay: Magnesium in zebrafish blood. Pachikian BD, et al (2010). Changes in intestinal bifidobacteria levels are associated with the inflammatory response in magnesium-deficient mice. J Nutr. 140(3):509-14. Assay: Magnesium in mice serum. LaRusso J, Li Q, Jiang Q, Uitto J (2009). Elevated dietary magnesium prevents connective tissue mineralization in a mouse model of pseudoxanthoma elasticum (Abcc6(-/-)). J Invest Dermatol 129:1388-1394. Assay: Magnesium in mouse serum, urine. Pratihar, S., & Kundu, J. K. (2009). Increased Serum Magnesium and Calcium and their Regulation During Hibernation in the Indian Common Toad, Duttaphrynus melanostictus (Schneider, 1799). South American Journal of Herpetology 4(1): 51-54. Assay: Magnesium in toad serum. Stippler, M et al (2007) Serum and Cerebrospinal Fluid Magnesium in Severe Traumatic Brain Injury Outcome. J Neurotrauma. 24(8): 1347-1354. Assay: Magnesium in human cerbrospinal fluid. Kim TW et al (2006) Rapid production of milligram quantities of proteins in a batch cell-free protein synthesis system. J Biotechnol 124(2):373-80. Assay: Magnesium in cell free system protein.To find more recent publications, pleaseclick here.