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主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
BioAssay Systems/EnzyChrom™ GSH/GSSG Assay Kit/100 tests/EGTT-100
产品编号:EGTT-100
市  场 价:¥5780.00
场      地:美国(厂家直采)
产品分类: 生化试剂>其他>>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$289.00
品      牌: BioAssay Systems
公      司:BioAssaySystems
公司分类:
BioAssay Systems/EnzyChrom™ GSH/GSSG Assay Kit/100 tests/EGTT-100
商品介绍

EnzyChrom™ GSH/GSSG Assay Kit

EnzyChrom™ GSH/GSSG Assay Kit Catalog No: EGTT-100
Price: $289 Qty:
For orders of 10 or more kits, please call +1-510-782-9988x1 oremail us for best pricing and/or bulk order. Shipping: On Ice Shipment: Fedex ServiceDelivery: 1-2 days (US), 3-6 days (Intl) Storage: -20°C
Glutathione Assay Kit (EGTT-100)
  • Product Overview
  • Product FAQ
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  • Assay Service
ProtocolSDS

Application

  • For quantitative determination of reduced and oxidized glutathione (GSH/GSSG).

Key Features

  • Sensitive and accurate. Linear detection range 0.01-3 µM GSH equivalents with a detection limit of 10 nM GSH equivalents.

Method

  • OD412nm

Samples

  • Whole blood, plasma, serum, urine, tissue and cell extracts

Species

  • All

Size

  • 100 tests

Detection Limit

  • 10 nM

Shelf Life

  • 6 months

More Details

  • Glutathione , a tripeptide of glycine, glutamic acid and cysteine, is one of the key antioxidants involved in protecting cells from damages by reactive oxygen species. Glutathione (GSH) reduces disulfide bonds in cytoplasmic proteins to cysteines, in which it is converted to its oxidized form GSSG. BioAssay Systems GSH/GSSG Assay Kit is designed to accurately measure total, reduced and oxidized glutathione in biological samples using an enzymatic method that utilizes Ellman’s Reagent (DTNB) and glutathione reductase (GR). DTNB reacts with reduced glutathione to form a yellow product. The rate of change in the optical density,measured at 412 nm, is directly proportional to glutathione concentration in the sample. This kit can also be used to measure oxidized (GSSG) by using a specific protocol which first scavenges all GSH with 1-methyl-2-vinylpyridinium triflate.
For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.
Lin, T. A., Ke, B. J., Cheng, C. S., Wang, J. J., Wei, B. L., & Lee, C. L. (2019). Red Quinoa Bran Extracts Protects against Carbon Tetrachloride-Induced Liver Injury and Fibrosis in Mice via Activation of Antioxidative Enzyme Systems and Blocking TGF-beta1 Pathway. Nutrients, 11(2), 395. Assay: GSH/GSSG in mice tissues. Kim, K. H., Kim, E. Y., Lee, S. Y., Ko, J. J., & Lee, K. A. (2018). Oocyte Cytoplasmic Gas6 and Heparan Sulfate (HS) are Required to Establish the Open Chromatin State in Nuclei During Remodeling and Reprogramming. Cellular Physiology and Biochemistry, 45(1), 37-53. Assay: GSH/GSSG in mice cells. Li, X. X., Wang, Z. J., Zheng, Y., Guan, Y. F., Yang, P. B., Chen, X. & Chien, K. Y. (2018). Nuclear receptor Nur77 facilitates melanoma cell survival under metabolic stress by protecting fatty acid oxidation. Molecular cell, 69(3), 480-492. Assay: GSH/GSSG in mice cells. Medina-Leendertz, S., Mora, M., Vielma, J. R., Bravo, Y., Atencio-Bracho, L., Leal-Yepez, A. & Bonilla, E. (2018). Melatonin decreases oxidative stress in Drosophila melanogaster exposed to manganese. Investigacion Clinica, 59(3), 230-241. Assay: GSH/GSSG in D. melanogaster tissues.Paul, S., Gangwar, A., Bhargava, K., & Ahmad, Y. (2018). STAT3-RXR-Nrf2 activates systemic redox and energy homeostasis upon steep decline in pO2 gradient. Redox biology, 14, 423-438. Assay: GSH/GSSG in rat tissues and plasma. Bonilla, E., Medina-Leendertz, S., Mora, M., Vielma, J. R., Atencio-Bracho, L., Bravo, Y. & Arcaya, J. L. (2017). Minocycline Protection against Paraquat Toxicity in Drosophila melanogaster. Toxicology International, 24(1), 65-73. Assay: GSH/GSSG in human tissues. Paul, S., Gangwar, A., Bhargava, K., & Ahmad, Y. (2017). Deciphering Molecular Cascades In A Novel Acclimatization Strategy For Rapid Ascent To High Altitude. bioRxiv, 145342. Assay: GSH/GSSG in rat tissues and plasma.Khatri, R., Krishnan, S., Roy, S., Chattopadhyay, S., Kumar, V., & Mukhopadhyay, A. (2016). Reactive oxygen species limit the ability of bone marrow stromal cells to support hematopoietic reconstitution in aging mice. Stem cells and development 25(12):948-58. Assay: GSH/GSSG in mice stromal cells. Lee, H. N., et al. (2015). Heme Oxygenase-1 Determines the Differential Response of Breast Cancer and Normal Cells to Piperlongumine. Molecules and cells, 38(4), 327. Assay: GSH/GSSG in piperlongumine cell. Chatterjee, N., Eom, H. J., & Choi, J. (2014). A systems toxicology approach to the surface functionality control of graphene-cell interactions. Biomaterials, 35(4), 1109-1127. Assay: GSH/GSSG in human HepG2 cells. Di Maro, G., et al. (2014). Anterior gradient protein 2 promotes survival, migration and invasion of papillary thyroid carcinoma cells. Molecular cancer, 13(1), 1. Assay: GSH/GSSG in human tissues. Muller, S., et al. (2014). Age-dependent effects of UCP2 deficiency on experimental acute pancreatitis in mice. PloS one, 9(4), e94494. Assay: GSH/GSSG in mice serum.To find more recent publications, pleaseclick here.
If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.Simply send us your samples:- Fast turnaround - Quality data - Low costPlease email or call 1-510-782-9988 x 2 to request assay service.
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BioAssay Systems开发和销售创新的高通量分析解决方案,以满足生命科学行业不断增长的需求。BioAssay Systems的创始人在现代药物发现领域拥有50多年的丰富经验。在过去的15年中,我们已经开发了200多种用于药物发现和生命科学研究的检测试剂盒。我们以提供的服务和简单,易用且性能卓越的产品而感到自豪。

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