EnzyChrom™ Ethanol Assay Kit

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ProtocolSDS

Application

For quantitative determination of ethanol and evaluation of drug effects on alcohol metabolism.

Key Features

Sensitive and accurate. Detection limit 0.0008 vol % (140 μM or 8 ppm), linearity up to 0.1% ethanol in 96-well plate assay.
Convenient. The procedure involves adding working reagent, incubating for 30 min and stopping reaction. No 37°C heater is needed.
High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

Method

OD565nm (Enzymatic)

Samples

Serum, plasma, urine, saliva samples etc

Species

Size

100 tests

Detection Limit

8.0E-6

Shelf Life

6 months

More Details

Alcoholic drinksare among the daily consumed beverages. Studies have shown heavy alcohol consumption may lead to various forms of liver diseases and to increased mortality rates. Quantitative determination of alcohol (ethanol, C₂H₅OH) has applications in basic research, drug discovery, clinic studies and in the alcoholic industry. Simple, direct and automation-ready procedures for measuring ethanol concentration are very desirable. BioAssay Systems EnzyChrom™ ethanol assay kit is based on alcohol dehydrogenase catalyzed oxidation of ethanol, in which the formed NADH is coupled to the formazan (MTT) chromogen. The intensity of the product color, measured at 565 nm, is proportionate to the ethanol concentration in the sample.

Does the assay react with methanol?

No, the assay does essentially not cross-react with methanol. Only at high concentrations (more than 100 mM methanol) will there be any significant nonspecific signal.For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.

Davis-Anderson, Katie L., et al (2018). Fetal regional brain protein signature in FASD rat model. Reproductive Toxicology 76: 84-92. Assay: Ethanol in rat plasma. Liu, Jie, et al (2018). Traumatic stress affects alcohol-drinking behavior through cocaine-and amphetamine-regulated transcript 55-102 in the paraventricular nucleus in rats. Molecular medicine reports 17.1: 1157-1165. Assay: Ethanol in rat serum. Miozzo, Federico, et al (2018). Alcohol exposure promotes DNA methyltransferase DNMT3A upregulation through reactive oxygen species-dependent mechanisms. Cell Stress and Chaperones 23.1: 115-126. Assay: Ethanol in mouse cells. Qiao, Xiaomeng, et al (2017). 5-Aza-2"-deoxycytidine in the medial prefrontal cortex regulates alcohol-related behavior and Ntf3-TrkC expression in rats. PloS one 12.6: e0179469. Assay: Ethanol in rat plasma. Zhou, Huiming, et al (2017). Alcohol exposure during pregnancy causes non-compaction cardiomyopathy in offspring mice. Journal of Third Military Medical University, 39(17): 1696-1701. Assay: Ethanol in mice serum.Sanny, Tony, and Benjamin C. Stark (2016). Increased ethanol production by genetic engineering of microorganisms to express hemoglobin. U.S. Patent No. 9,309,540. Assay: Ethanol in microorganisms cell cuture.Echevarria DJ, et al. (2011). Alcohol-induced behavior change in zebrafish models. Rev Neurosci. 22(1):85-93. Assay: Ethanol in zebrafish blood. Magne L, et al. (2011). ATF4 and the integrated stress response are induced by ethanol and cytochrome P450 2E1 in human hepatocytes. J Hepatol. 54(4):729-37. Assay: Ethanol in human hepatocytes. Echevarria,DJ and Hammack,CH (2010). Does acute alcohol exposure modulate aggressive behaviors in the zebrafish (Danio rerio), or is the bark worse than the bite. ijcp 23:62-69. Assay: Ethanol in zebrafish blood.Ou XM, et al. (2010). A novel role for glyceraldehyde-3-phosphate dehydrogenase and monoamine oxidase B cascade in ethanol-induced cellular damage. Biol Psychiatry 67(9):855-63. Assay: Ethanol in human neuroblastoma cell. Suwannarangsee S, et al. (2010). Characterization of alcohol dehydrogenase 1 of the thermotolerant methylotrophic yeast Hansenula polymorpha. Appl Microbiol Biotechnol. 88(2):497-507. Assay: Ethanol in yeast cell. Pariset, JJ. al (2009). Alcohol Dose-Dependently Enhances 3 alpha-Androstanediol Formation in Frontal Cortex of Male rats Concomitant with Aggression. Open Neuropsychopharmacology 2:1-10. Assay: Ethanol in rat blood.Jeong WI et al (2008). Paracrine activation of hepatic CB1 receptors by stellate cell-derived endocannabinoids mediates alcoholic fatty liver. Cell Metab. 7(3):227-35. Assay: Ethanol in mouse serum.To find more recent publications, pleaseclick here.

If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.Simply send us your samples:- Fast turnaround - Quality data - Low costPlease email or call 1-510-782-9988 x 2 to request assay service.

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