QuantiFluo™ Caspase-3 Assay Kit

Application

• For quantitative determination of the apoptosis target caspase-3 activity and HTS screen for apoptosis inducers and inhibitors.

Key Features

• Safe. Non-radioactive assay.
• Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method

• FL400/490nm

Samples

• Cell and tissue lysate

Species

• All

Size

• 100 tests

Shelf Life

• 12 months

More Details

• Caspases are members of the aspartate-specific cysteinyl protease family that play a central role in apoptosis. Apoptosis is involved in a variety of physiological and pathological events, ranging from normal fetal development to diseases such as cancer, organ failure, and neurodegenerative diseases. Caspase-3 is key biomarker in the assessment of apoptosis and in understanding mechanism of apoptosis induction. BioAssay Systems Caspase-3 Assay Kit provides a convenient means to measure caspase-3 activity in biological samples. In the assay, a specific substrate (N-Ac-DEVD-AFC) is cleaved by active caspase-3, forming a highly fluorescent product.The fluorescence intensity (λex/em = 400/490nm) is proportional to the caspase-3 activity.