QuantiFluo™ DNA Assay Kit

Application

• For quantitative determination of DNA.

Key Features

• Sensitive and accurate. Linear detection range 2 ng to 40 ng (100 - 2,000 ng/mL) calf thymus DNA in 96-well plate assay.
• Simple and high-throughput. The "mix-and-read" procedure involves addition of a single working reagent and reading the fluorescence intensity. Can be readily automated as a high-throughput assay for thousands of samples per day.
• Low interference. RNA, salt (up to 3M NaCl), detergent (< 0.01%="" sds)="" and="" common="" dna="" extraction="" buffer="" do="" not="" interfere="" in="" the="">

Method

• FL340/450nm

Samples

• Plasmid DNA, genomic DNA, cDNA, DNA following polymerase chain reaction, and DNA extracted from gel and other matrices

Species

• All

Size

• 250 tests

Detection Limit

• 100 ng/mL

Shelf Life

• 12 months

More Details

• DNA quantitation is a common practice in molecular biology. Very often DNA is available in minute quantities and the traditional UV 260 nm absorbance method requires microgram quantities for reliable results. Accurate determination of DNA concentration, especially when DNA is present at low concentrations, is crucial for reproducible results in sequencing, cloning, transfection and DNA labeling. Simple, direct and automation-ready procedures for measuring DNA concentration are very desirable. BioAssay Systems QuantiFluo™ DNA assay kit is designed to accurately measure nanogram quantities of plasmid DNA, cDNA, DNA following polymerase chain reaction and DNA eluted from gels. The improved method utilizes Hoechst dye that binds specifically with double-stranded DNA. The fluorescence intensity, measured at λex/em = 350/450nm, is directly proportional to the DNA concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples.