QuantiChrom™ Indole Assay Kit

Product Overview
Product FAQ
Product Citations
Assay Service

ProtocolSDS

Application

Indole determination in biological samples

Key Features

Fast and sensitive. Use of 100µL sample. Linear detection range from 3 to 100 µM indole in 96-well plate assay.
Convenient. The procedure involves adding a single working reagent, and reading the absorbance immediately.

Method

OD565nm

Samples

Bacterial growth media and other biological samples

Species

Size

100 tests

Detection Limit

3 µM

Shelf Life

6 months

More Details

INDOLE is the primary product of tryptophan breakdown by tryptophanase. The indole test is commonly performed on bacteria to classify them on their ability to break down tryptophan to indole. BioAssay Systems indole assay kit is based on a modified version of Ehlrich"s and Kovac"s reagents, which reacts with indole to produce a colored compound at 565 nm. The intensity of this colored compound is directly proportional to the indole in the sample.

Does the assay work with urine samples

The Assay will detect indole in urine samples; however, the reagent also reacts with urobilinogen in the urine. It does not distinguish between the two.

Does the assay work with serum samples?

The acidity of the reagent often causes serum samples to precipitate which greatly interferes with the assay.

Do I need to read the OD immediately after adding the reagent?

Yes, we recommend you read the OD shortly after adding the reagent to the samples. The OD of the reaction mixture slowly decreases over time. We would recommend reading the OD within 10 minutes of adding the reagent.For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.

No citations for this new product. Please check back later.You mayclick here to check if citations are available, but are not listed here yet.

If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.Simply send us your samples:- Fast turnaround - Quality data - Low costPlease email or call 1-510-782-9988 x 2 to request assay service.

品牌介绍

BioAssay Systems开发和销售创新的高通量分析解决方案，以满足生命科学行业不断增长的需求。BioAssay Systems的创始人在现代药物发现领域拥有50多年的丰富经验。在过去的15年中，我们已经开发了200多种用于药物发现和生命科学研究的检测试剂盒。我们以提供的服务和简单，易用且性能卓越的产品而感到自豪。

公司介绍

更多...

产品分类

更多...