QuantiQuik™ D-Lactic Acid Quick Test Strips

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ProtocolSDS

Application

Direct determination of D-lactic acid concentrations in food and beverage samples as well as biological samples.

Key Features

Fast and sensitive. Use of 20 or 100 µL sample. Semi-quantitative measurement between 0-180 mg/L (undiluted) D-lactic acid.
Convenient. No expensive lab equipment needed.
Sample treatment and assay can be performed in under 15 minutes.

Method

Visual

Samples

Wine, beer, milk, etc

Species

Size

10 tests

Shelf Life

6 months

More Details

LACTIC ACID, or lactate, is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. D-lactate is produced in only minor quantities in animals and measuring for D-lactate in animal samples is a means to determine the presence of bacterial infection. Furthermore, since D-lactate is a specific indicator of bacteria fermentation, its measurement can be used to determine the freshness of milk, meat and fruit juices. Elevated levels of D-lactate in wine is an indication of lactic acid bacteria contamination. BioAssay Systems QuantiQuik™ D-Lactate Test Strips are based on D-Lactate dehydrogenase catalyzed oxidation of D-lactate in which the formed NADH reduces a chromogenic reagent. The intensity of product color, is directly proportional to D-lactate concentration in the sample.

What samples have you tested?

The strips have been tested on red wine, white wine, homogenized whole milk, cultured buttermilk, yogurt, malt mash, beer and lemonade.

My sample turns the strip very dark purple, how can I determine the D-lactate concentration?

Very dark purple indicates that the diluted sample concentration is greater than 180 mg/L. To obtain a more accurate concentration, the sample should be further diluted and retested. For example, if the sample was diluted 5×, try diluting 21×.

I don’t have access to a pipetteman. How can I accurately measure out my samples?

We offer exact volume transfer pipettes as an accessory. For samples requiring a 21× dilution, order the 20 µL transfer pipettes (TP20). For samples requiring a 5× dilution, order the 100 µL transfer pipettes (TP100).

Can I store unused reagents for future use?

Yes, unused reagents can be stored according to the assay protocol. The strips should be kept in a dry, cool location and protected from light.For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.

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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.Simply send us your samples:- Fast turnaround - Quality data - Low costPlease email or call 1-510-782-9988 x 2 to request assay service.

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