EnzyChrom™ Creatine Assay Kit

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ProtocolSDS

Application

For quantitative determination of creatine and evaluation of drug effects on creatine metabolism.

Key Features

High sensitivity and wide linear range. Use 10 μL sample. Linear detection range 4 to 1000 μM (colorimetric) or 0.5 to 50 μM (fluorimetric).
Homogeneous and simple procedure. Simple "mix-and-measure" procedure allows reliable quantitation of creatine within 30 minutes.

Method

OD570nm, or FL530/585nm

Samples

Serum, plasma, urine, saliva etc

Species

Size

100 tests

Detection Limit

4 μM

Shelf Life

12 months

More Details

CREATINE is present in vertebrates and helps to supply energy to muscle. In humans and animals, approximately half of creatine originates from food (mainly from fresh meat). Creatine supplementation has been investigated as a possible therapeutic approach for the treatment of muscular, neuromuscular, neurological and neurodegenerative diseases. Simple, direct and automation-ready procedures for measuring creatine are popular in research and drug discovery. BioAssay Systems creatine assay is based on enzymatic reactions leading to formation of a pink colored product. The optical density at 570 nm or fluorescence intensity at λ ex/em = 530/585 nm is directly proportional to the creatine concentration in the sample.

Can this kit be used to measure creatine concentrations in cells?

Yes. To determine intracellular creatine concentrations, harvest cells (0.1-2 million cells per assay) in a 1.5mL tube, centrifuge 1-2 min at 3,000 rpm on a table centrifuge. Remove culture medium, wash cells quickly with cold PBS. Immediately remove any PBS. Prepare Working Reagent. Add 120 µL WR directly to the cell pellet and 80 µL WR to standards in 96-wells. Vortex cell sample tube 1 min, incubate 30 min at room temp. Centrifuge 5 min at 14,000 rpm. Transfer 100 µL supernatant to 96-well. Read OD570nm on a plate reader. Notes: 1. cell number. It is prudent to run several doses of cells, e.g. 0.1, 1, 2×10⁶ cells to determine optimal cell number to be used in subsequent assays. Working Reagent should contain a lysis reagent. BioAssay Systems will supply this reagent upon request.For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.

Wang, Yujie, et al (2018). Guanidinoacetic Acid Regulates Myogenic Differentiation and Muscle Growth Through miR-133a-3p and miR-1a-3p Co-mediated Akt/mTOR/S6K Signaling Pathway. International journal of molecular sciences 19.9: 2837. Assay: Creatine in murine cells. Chan, Yiumo Michael, et al (2017). Substantial deficiency of free sialic acid in muscles of patients with GNE myopathy and in a mouse model. PloS one 12.3: e0173261. Assay: Creatine in human and mouse tissues. Holz, Josefin-Beate, and Alex Hemeryck (2017). Inhibition of bone resorption with rankl binding peptides. U.S. Patent Application No. 15/295,229. Assay: Creatine in human urine.Schoborg, JA et al (2014). Substrate replenishment and byproduct removal improve yeast cell-free protein synthesis. Biotechnology Journal 9(5): 630-640. Assay: Creatine in yeast cells. Qi S et al (2012). Comparison of the metabolic profiling of hepatitis B virus-infected cirrhosis and alcoholic cirrhosis patients by using (1) H NMR-based metabonomics. Hepatol Res 42(7):677-85. Assay: Creatine in human serum.To find more recent publications, pleaseclick here.

If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.Simply send us your samples:- Fast turnaround - Quality data - Low costPlease email or call 1-510-782-9988 x 2 to request assay service.

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BioAssay Systems开发和销售创新的高通量分析解决方案，以满足生命科学行业不断增长的需求。BioAssay Systems的创始人在现代药物发现领域拥有50多年的丰富经验。在过去的15年中，我们已经开发了200多种用于药物发现和生命科学研究的检测试剂盒。我们以提供的服务和简单，易用且性能卓越的产品而感到自豪。

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