What are the differences between the three EnzyChrom Glucose assay kits?
EBGL - best at detecting low levels of glucose (as low as 5 µM)EGL2 - best at measuring high levels of glucose using a low wavelength filter (340 nm)EGL3 - best at measuring high levels of glucose using a high wavelength filter (565 nm)Which of the three EnzyChrom Glucose assay kits can be used with urine, saliva, or serum samples?
EBGL - Not recommended for urine, saliva, or serum samplesEGL2 - Compatible with urine and serum samples, not recommended for saliva samplesEGL3 - Recommended for urine, saliva, and serum samples*Sample blanks are to be used when testing urine samples with EGL2 or EGL3How specific is this assay (EBGL)?
Glucose oxidase is fairly specific for D-glucose and does not react with fructose, galactose, or xylose. It does react with the following sugars: 2-deoxy-D-glucose (11% relative activity compared to D-glucose), 4-o-methyl-D-glucose (7%) and mannose (3%).Can this kit be used to measure glucose concentrations in cells?
Glucose is converted into glucose-6-phosphate within the cell, which may not react with our assay. We have not tested it, but if the concentration of free glucose in the cell is within the detection range (1 to 30 μM for fluorimetric assays) of the assay it could still work.For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.