EnzyChrom™ Citrate Assay Kit

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ProtocolSDS

Application

For quantitative determination of citrate or citric acid and evaluation of drug effects on citrate metabolism.

Key Features

Fast and sensitive. Linear detection range: 4 to 400 μM citrate for colorimetric assays and 0.5 to 40 μM for fluorimetric assays.
Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method

OD570nm, or FL530/585nm

Samples

Serum, plasma, agriculture etc

Species

Size

100 tests

Detection Limit

OD, FL: 4, 0.5 μM.

Shelf Life

6 months

More Details

CITRATE is an intermediate in the citric acid cycle and is involved in fatty acid synthesis. BioAssay Systems' Citrate Assay Kit provides a simple, and automation-ready procedure for measuring citrate concentration. Citrate is converted into pyruvate which is then oxidized with the conversion of the dye into a colored and fluorescent form. The color intensity at 570 nm or fluorescence intensity at λex/em = 530/585 nm is directly proportional to the citrate concentration in the sample.

For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.

Fu, Xuekun, et al (2018). Runx2/Osterix and zinc uptake synergize to orchestrate osteogenic differentiation and citrate containing bone apatite formation. Advanced Science 5.4: 1700755. Assay: Citrate in mice cells. Trivedi, Amit Kumar, et al. (2015). Adaptation of oxidative phosphorylation to photoperiod-induced seasonal metabolic states in migratory songbirds. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology 184): 34-40. Assay: Citrate in bird plasma.To find more recent publications, pleaseclick here.

If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.Simply send us your samples:- Fast turnaround - Quality data - Low costPlease email or call 1-510-782-9988 x 2 to request assay service.

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BioAssay Systems开发和销售创新的高通量分析解决方案，以满足生命科学行业不断增长的需求。BioAssay Systems的创始人在现代药物发现领域拥有50多年的丰富经验。在过去的15年中，我们已经开发了200多种用于药物发现和生命科学研究的检测试剂盒。我们以提供的服务和简单，易用且性能卓越的产品而感到自豪。

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