EnzyChrom™ Ascorbic Acid Assay Kit

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ProtocolSDS

Application

For quantitative determination of ascorbate (ascorbic acid) and evaluation of drug effects on ascorbic acid metabolism.

Key Features

Use 20 μL samples. Linear detection range: colorimetric assay 6 to 1000 μM, fluorimetric assay 1 to 100 μM ascorbic acid.

Method

OD570nm, or FL530/585nm

Samples

Serum, plasma, urine, saliva, milk, tissue, and cell culture

Species

Size

100 tests

Detection Limit

OD, FL: 6, 1 μM

Shelf Life

6 months

More Details

Ascorbic acid (the L-enantiomer commonly known as vitamin C) is an important antioxidant found in living organisms and applied as additives in food and other industrial processes. By reacting with reactive oxygen species, it protects the cell from oxidative damages. BioAssay Systems method provides a simple, direct and high-throughput assay for measuring ascorbic acid. In this assay, ascorbic acid is oxidized by ascorbate oxidase resulting in the production of H₂O₂ which reacts with a specific dye to form a pink colored product. The color intensity at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the ascorbic acid concentration in the sample.

We would like to measure ascorbic acid in liver homogenates, which are in 0.25M Sucrose. We want to confirm the sucrose will interfere with the assay?

We have not tested the compatibility of sucrose with this assay. Based on the mechanism of the assay we see no reason why sucrose would interfere and believe the assay will work just fine.For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.

Susanto, L., Y. Siregar, and L. Kusumawati (2018). Vitamin C supplementation improve the sputum conversion culture rate in pulmonary tuberculosis treatment while rifampicin susceptible. IOP Conference Series: Earth and Environmental Science 125(1). Assay: Ascorbic acid in human plasma.Kantar, Michael B., et al (2016). Vitamin variation in Capsicum Spp. provides opportunities to improve nutritional value of human diets. PloS one 11.8: e0161464. Assay: Ascorbic acid in pepper tissue. Trezzi, Jean-Pierre, et al (2016). LacaScore: a novel plasma sample quality control tool based on ascorbic acid and lactic acid levels. Metabolomics 12.6: 96. Assay: Ascorbic acid in human plasma. Sasaki, M. et al (2012). D-2-hydroxyglutarate produced by mutant IDH1 perturbs collagen maturation and basement membrane function. Genes & Dev. 26: 2038-2049. Assay: Ascorbic acid in mouse cell. Ching-Shih Chen et al (2011). Thiazolidinedione Energy Restriction-Mimetic Agents. US 2011/0086895 Al. Assay: Ascorbic acid in mouse cell. To find more recent publications, pleaseclick here.

If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.Simply send us your samples:- Fast turnaround - Quality data - Low costPlease email or call 1-510-782-9988 x 2 to request assay service.

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BioAssay Systems开发和销售创新的高通量分析解决方案，以满足生命科学行业不断增长的需求。BioAssay Systems的创始人在现代药物发现领域拥有50多年的丰富经验。在过去的15年中，我们已经开发了200多种用于药物发现和生命科学研究的检测试剂盒。我们以提供的服务和简单，易用且性能卓越的产品而感到自豪。

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