QuantiFluo™ Ammonia Assay Kit

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ProtocolSDS

Application

For quantitative determination of ammonia/ammonium concentration by fluorimetry at 360nm/450nm (compatible on handheld fluorimeter catalog# FL360450).

Key Features

Fast and sensitive. Linear detection range of 0.012 - 1 mM ammonia.
Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method

FL360/450nm

Samples

Biological (eg urine) and environ-mental samples

Species

Size

200 tests

Detection Limit

12 μM

Shelf Life

6 months

More Details

AMMONIA(NH₃) or its ion form ammonium (NH₄⁺) is an important source of nitrogen for living systems and is ubiquitously present in the nature. Simple, direct and automation-ready procedures for measuring NH₃ are very desirable. BioAssay Systems ammonia/ammonium assay is based on an improved o-phthalaldehyde method. This reagent reacts with ammonia/ ammonium and forms a fluorescent product. The fluorescence intensity (λex/em = 360/450nm) is proportional to the ammonia concentration in the sample.

For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.

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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.Simply send us your samples:- Fast turnaround - Quality data - Low costPlease email or call 1-510-782-9988 x 2 to request assay service.

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BioAssay Systems开发和销售创新的高通量分析解决方案，以满足生命科学行业不断增长的需求。BioAssay Systems的创始人在现代药物发现领域拥有50多年的丰富经验。在过去的15年中，我们已经开发了200多种用于药物发现和生命科学研究的检测试剂盒。我们以提供的服务和简单，易用且性能卓越的产品而感到自豪。

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