QuantiChrom™ Carbonyl Assay Kit

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ProtocolSDS

Application

Quantification of carbonyl groups or protein carbonyls in biological samples.

Key Features

Fast and sensitive. Use of 100 µL sample. Linear detection range from 12 to 250 µM carbonyl in 96-well plate assay.
Convenient. The procedure involves adding a single working reagent, and reading the absorbance after 30 min.
High-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method

OD375nm

Samples

Chemicals with carbonyl groups (e.g. ketones, aldehydes) or protein carbonyls in biological samples (e.g. oxidized BSA, etc.)

Species

Size

100 tests

Detection Limit

12 µM

Shelf Life

12 months

More Details

CARBONYL groups, such as ketones and aldehydes, are a commonindicator of protein oxidation. Protein oxidation is caused as a result of exposure to reactive oxygen species (ROS) and is a common marker in various diseases as well as aging. BioAssay Systems' Carbonyl Assay Kit is based on an improved method, where 2,4-dinitrophenylhydrazine (DNPH) reacts with carbonyl groups to produce a colored compound at 375 nm. The intensity of this colored compound is directly proportional to the carbonyl groups in the sample.

The background is very high. Is this normal?

The background absorbance is typically ~ 0.5. This is completely normal.

Can I store the diluted standards (250 µM, 150 µM, 75 µM, and 0 µM) to use for later?

We recommend preparing the standards fresh from the 50 mM each day.

Do I need to run a standard curve with every assay?

Yes.

How should I store the kit?

The kit is shipped at room temperature. Upon delivery, store at -20°C.

Precipitate formed when I added the reagent to the sample. What should I do?

The Reagent is acidic and may cause precipitation in samples that have significant amounts of protein. You may need to dilute your sample, repeat the assay, and correct for the dilution factor in the calculation.

What is the Carbonyl Standard?

The standard is pyruvate, which has two carbonyl groups per molecule. So the 50 mM Carbonyl Standard is actually 25 mM pyruvate.

My sample has high levels of nucleic acids. Will this interfere?

Nucleic acids can artificially increase the carbonyl count if they are present in high levels. If the ratio of 280/260 nm is less than 1, samples should be mixed with 1% streptomycin sulfate to denature the nucleic acids. Mix well, incubate the samples at room temperature for 15 minutes and then centrifuge at 5000xg for 10 minutes. Use the supernatant in the assay.For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.

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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.Simply send us your samples:- Fast turnaround - Quality data - Low costPlease email or call 1-510-782-9988 x 2 to request assay service.

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BioAssay Systems开发和销售创新的高通量分析解决方案，以满足生命科学行业不断增长的需求。BioAssay Systems的创始人在现代药物发现领域拥有50多年的丰富经验。在过去的15年中，我们已经开发了200多种用于药物发现和生命科学研究的检测试剂盒。我们以提供的服务和简单，易用且性能卓越的产品而感到自豪。

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