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主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
BioAssay Systems/EnzyChrom™ Acetaldehyde Assay Kit/100 tests/EACT-100
产品编号:EACT-100
市  场 价:¥5380.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$269.00
品      牌: BioAssay Systems
公      司:BioAssaySystems
公司分类:
BioAssay Systems/EnzyChrom™ Acetaldehyde Assay Kit/100 tests/EACT-100
商品介绍

EnzyChrom™ Acetaldehyde Assay Kit

EnzyChrom™ Acetaldehyde Assay Kit Catalog No: EACT-100
Price: $269 Qty:
For orders of 10 or more kits, please call +1-510-782-9988x1 oremail us for best pricing and/or bulk order. Shipping: On Ice Shipment: Fedex ServiceDelivery: 1-2 days (US), 3-6 days (Intl) Storage: -20°C
Colorimetric Acetaldehyde Assay Kit
  • Product Overview
  • Product FAQ
  • Product Citations
  • Assay Service
ProtocolSDS

Application

  • Acetaldehyde determination in biological and food/beverage samples.

Key Features

  • Fast and sensitive. Linear detection range (20µL sample): 2µM to 2 mM acetaldehyde in 96-well plate assay.
  • High-throughput.The procedure involves adding a single working reagent, and reading the absorbance after 30 minutes. Room temperature assay. No 37°C heater is needed.
  • Convenient.Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method

  • OD565nm

Samples

  • Biological samples (e.g. plasma, serum, urine, tissue and culture media) and food/beverage samples (e.g. wine, coffee, and juice).

Species

  • All

Size

  • 100 tests

Detection Limit

  • 2 µM

Shelf Life

  • 6 months

More Details

  • ACETALDEHYDE (CH3CHO) is one of the most widely occurring aldehydes in nature and is commonly used in industry. Acetaldehyde, a metabolic byproduct of ethanol in the liver, is toxic to the human body and is rapidly converted to the less harmful acetic acid by the enzyme aldehyde dehydrogenase. People with a deficiency of aldehyde dehydrogenase accumulate acetaldehyde when consuming alcohol and this accumulation results in facial and body flushing often referred to as “Asian flush syndrome.” Build up of acetaldehyde has also been associated with the effects of hangovers from alcohol consumption. Although classified as a carcinogen, acetaldehyde is naturally found in many foods and beverages such as ripe fruit, coffee, and wine. BioAssay Systems colorimetric acetaldehyde assay kit is based on aldehyde dehydrogenase catalyzed oxidation of acetaldehyde, in which the formed NADH reduces a formazan reagent. The intensity of the product color, measured at 565 nm, is directly proportional to the acetaldehyde concentration in the sample.

How specific is this assay for acetaldehyde?

The assay mechanism uses the enzyme aldehyde dehydrogenase to quantify acetaldehyde in the sample. The enzyme will also oxidize other aldehydes in the sample (i.e. propionaldehyde). Therefore, the assay will not distinguish between acetaldehyde and other aldehydes present in the sample. Consideration should be taken if high levels of other aldehydes are expected in the sample being tested.

How long are the diluted standards good for?

Acetaldehyde is a rather volatile chemical, so proper storage of the standard is vital. Only bring the 3 M Standard to room temperature for time needed to prepare standards. Return to -20°C within 30 minutes of thawing. Be sure to keep the Standard and standard dilutions on ice or refrigerated with the tube caps closed during assay procedure. The diluted standards for the colorimetric assay (4 mM, 2.4 mM, 1.2 mM, and 0 mM) and the fluorimetric assay (60 µM, 36 µM, 18 µM, and 0 µM) should be prepared fresh before each assay and used within 10 minutes of preparing.

What temperature do I run the assay at?

The assay may be run at room temperature (25°C) or 37°C.For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.
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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.Simply send us your samples:- Fast turnaround - Quality data - Low costPlease email or call 1-510-782-9988 x 2 to request assay service.
品牌介绍

BioAssay Systems开发和销售创新的高通量分析解决方案,以满足生命科学行业不断增长的需求。BioAssay Systems的创始人在现代药物发现领域拥有50多年的丰富经验。在过去的15年中,我们已经开发了200多种用于药物发现和生命科学研究的检测试剂盒。我们以提供的服务和简单,易用且性能卓越的产品而感到自豪。

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