How do I store the kit?
This kit is shipped at room temperature. Upon receiving, please store the substrate at -20°C and the remainder of the kit in the refrigerator (4°C).How do I prepare cell samples for assays?
Collect cells by centrifugation at 2,000 x g for 5 min at 4°C. For adherent cells, do not harvest cells using proteolytic enzymes; rather use a rubber policeman. Homogenize (10-20 passes in a Dounce homogenizer on ice) or sonicate cells (preferably performed in an ice water bath) in an appropriate volume of cold PBS, approximately one million cells per mL. Centrifuge at 14,000 x g for 10 min at 4°C. Remove supernatant for assay. It is prudent to run a pilot test of the sample at different dilutions. Choose a dilution with the readings in the linear range of the standard curve for further assays. Most samples can be stored at -80°C if not assayed immediately.Do I need to use a standard or standard curve with each assay run?
Yes, it is highly recommended.For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.