QuantiChrom™ Arginase Assay Kit

What are the best samples, serum or plasma? And with or without heparin?

Wash 5-10 x10⁵ cells and resuspend cell pellet in 100 μL lysis buffer containing 0.4% Triton X-100, 25 mM Tris-HCl (pH 7.5) and 1 mM MnSO₄ (10 μL of the provided Mn Solution). Optionally, activate arginase by heating for 10 min at 55-60°C. For serum samples we found the heating step unnecessary, but we have not tested cell or tissue samples. Centrifuge for 2 minutes at 14,000 rpm on a table centrifuge and use clear supernatant in arginase assay.

I see in your protocol that you don’t heat the sample what is normally done in the literature. Is you protocol different? Should I heat my samples to activate the enzyme?

We tried to heat the enzyme (purified enzyme and serum samples) in the assay buffer and found no significant difference as compared to no heating. Therefore our protocol does not include the heating step.For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.

Zhao. J et al (2016). Botanical Drug Puerarin Attenuates 6-Hydroxydopamine(6-OHDA)-Induced Neurotoxicity via UpregulatingMitochondrial Enzyme Arginase-2. Mol Neurobiol. 53(4):2200-11. Assay: Arginase in rat pheochromocytoma cells. Johnson, FK et al (2015). Arginase promotes endothelial dysfunction and hypertension in obese rats. Obesity (Silver Spring). 23(2):383-90. Assay: Arginase in obese and lean zucker rats plasma. Csoka, B et al (2014). A2B Adenosine Receptors Prevent Insulin Resistance by Inhibiting Adipose Tissue Inflammation via Maintaining Alternative Macrophage Activation. Diabetes. 63(3):850-66. Assay: Arginase in rat adipose tissue. Nishio, H et al (2014). Immunosuppression through constitutively activated NF-kB signalling in human ovarian cancer and its reversal by an NF-kB inhibitor. Br J Cancer. 110(12):2965-74. Assay: Arginase in human epithelial cells. Rodriguez, MP et al (2014). Role of Metabolic Environment on Nitric Oxide Mediated Inhibition of Neointimal Hyperplasia in Type 1 and Type 2 Diabetes. Nitric Oxide. 36:67-75. Assay: Arginase in lean zucker rats plasma. Anderson JT, et al (2011). Elevated levels of NO are localized to distal airways in asthma. Free Radic Biol Med. 50(11):1679-88. Assay: Arginase in human bronchial fluids. Hegde S, et al (2011). human NKT cells direct the differentiation of myeloid APCs that regulate T cell responses via expression of programmed cell death ligands. J Autoimmun. 37(1):28-38. Assay: Arginase in human cells. Kennedy DJ, et al (2011). A CD36-dependent pathway enhances macrophage and adipose tissue inflammation and impairs insulin signalling. Cardiovasc Res. 89(3):604-13. Assay: Arginase in mouse plasma. Lechner MG, et al (2011). Functional characterization of human Cd33+ And Cd11b+ myeloid-derived suppressor cell subsets induced from peripheral blood mononuclear cells co-cultured with a diverse set of human tumor cell lines. J Transl Med. 9:90. Assay: Arginase in human cells. Ma, G et al (2011). Paired immunoglobin-like receptor-B regulates the suppressive function and fate of myeloid-derived suppressor cells. Immunity 34(3):385-95. Assay: Arginase in mouse macrophages. Riley E, et al (2011). Inhibition profile of Leishmania mexicana arginase reveals differences with human arginase I. Int J Parasitol. 41(5):545-52. Assay: Arginase in human protein extracts. Eruslanov E, et al (2010). Pivotal Advance: Tumor-mediated induction of myeloid-derived suppressor cells and M2-polarized macrophages by altering intracellular PGE2 catabolism in myeloid cells. J Leukoc Biol.88(5):839-48. Assay: Arginase in macrophages. Feola DJ, et al (2010). Azithromycin alters macrophage phenotype and pulmonary compartmentalization during lung infection with Pseudomonas. Antimicrob Agents Chemother. 54(6):2437-47. Assay: Arginase in mouse cell lysates. Sripadi P, et al (2010). Direct detection of diverse metabolic changes in virally transformed and tax-expressing cells by mass spectrometry. PLoS One 5(9):e12590. Assay: Arginase in human cell line. Wang D, et al (2010). Elevated mitochondrial reactive oxygen species generation affects the immune response via hypoxia-inducible factor-1alpha in long-lived Mclk1+/- mouse mutants. J Immunol. 184(2):582-90. Assay: Arginase in mouse tissue. Capuano G, et al (2009). Modulators of arginine metabolism support cancer immunosurveillance. BMC Immunol. 10:1. Assay: Arginase in mouse CD11b+ cells. Eruslanov E, et al (2009). Altered expression of 15-hydroxyprostaglandin dehydrogenase in tumor-infiltrated CD11b myeloid cells: a mechanism for immune evasion in cancer. J Immunol. 182(12):7548-57. Assay: Arginase in mouse cell lysates. Weiss, JM et al (2009). Successful immunotherapy with IL-2/anti-CD40 induces the chemokine-mediated mitigation of an immunosuppressive tumor microenvironment. PNAS 106(46):19455-60. Assay: Arginase in mouse tumor tissue. Bao B, et al (2008). Zinc supplementation decreases oxidative stress, incidence of infection, and generation of inflammatory cytokines in sickle cell disease patients. Transl Res. 152(2):67-80. Assay: Arginase in human tissue. Moertel L, et al (2008). Comparative real-time PCR and enzyme analysis of selected gender-associated molecules in Schistosoma japonicum. Parasitology 135(5):575-83. Assay: Arginase in snail protein extracts. Murphy BS, et al (2008). Azithromycin alters macrophage phenotype. J Antimicrob Chemother. 61(3):554-60. Assay: Arginase in human cells. Schwacha MG, et al (2008). Burn injury-induced alterations in wound inflammation and healing are associated with suppressed hypoxia inducible factor-1alpha expression. Mol Med. 14(9-10):628-33. Assay: Arginase in mouse wound homogenates.To find more recent publications, pleaseclick here.