QuantiChrom™ Antioxidant Assay Kit

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ProtocolSDS

Application

For quantitative determination of total antioxidant capacity (TAC) and evaluation of drug effects on TAC metabolism.

Key Features

Sensitive and accurate. Use 20 μL sample. Linear detection range from 1.5 to 1000 μM Trolox equivalents.
Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 10 min. Can be readily automated as a high-throughput assay for thousands of samples per day.

Method

OD570nm

Samples

Serum, plasma, urine, saliva, food and beverage etc

Species

Size

100 tests

Detection Limit

1.5 μM

Shelf Life

6 months

More Details

An ANTIOXIDANTis a molecule capable of slowing or preventing the oxidation of other molecules. Antioxidants protect the cells from damages by reactive oxygen species which are produced in oxidation reactions in the cell. Antioxidants can be small molecules such as glutathione, vitamins, or macromolecules such as catalase, glutathione peroxidase. As oxidative stress contributes to the development of many diseases including Alzheimer’s disease, Parkinson’s disease, diabetes, rheumatoid arthritis and neurodegeneration, the use of antioxidants in pharmacology is intensively studied. Antioxidants are also widely used as dietary supplements and in industry as preservatives in food, cosmetics, rubber and gasoline. Simple, direct and high-throughput assays for total antioxidant capacity (TAC) find wide applications in research, food industry and drug discovery. BioAssay Systems improved assay measures total antioxidant capacity in which Cu²⁺ is reduced by antioxidant to Cu⁺. The resulting Cu⁺ specifically forms a colored complex with a dye reagent. The color intensity at 570nm is proportional to TAC in the sample.

What substances are known to interfere with the antioxidant assay?

Detergents, such as TWEEN-20, TRITON X-100, NP-40, should not be present at any concentration in the test sample. Reducing materials (DTT, 2-mercaptoethanol) and metal chelators (e.g. EDTA) may interfere with the assay.

How to determine TAC in tissue samples?

Homogenize tissue samples on ice in cold 1xPBS (~100 mg of tissue/0.5 ml of buffer) and then centrifuge at 12,000g for 15 minutes at 4°C. Remove the supernatant and keep it on ice. For long term storage, store in working aliquots at –80°C.For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.

Indrastiti, R. K., Rahmayanti, F., & Soegyanto, A. I. (2019). Analysis of the Total Antioxidant Capacity of Saliva in Smokers. Pesquisa Brasileira em Odontopediatria e Clinica Integrada, 19(1), 4897. Assay: Antioxidant in human saliva.Moore, M., Moriarty, T. A., Connolly, G., Mermier, C., Amorim, F., Miller, K., & Zuhl, M. (2019). Oral Glutamine Supplement Reduces Subjective Fatigue ratings during Repeated Bouts of Firefighting Simulations. Safety, 5(2), 38. Assay: Antioxidant in horseshoe crab serum. Neves, R. V. P., Rosa, T. S., Souza, M. K., Oliveira, A. J. C., Gomes, G. N. S., Brixi, B. & Prestes, J. (2019). Dynamic, Not Isometric Resistance Training Improves Muscle Inflammation, Oxidative Stress and Hypertrophy in rats. Frontiers in physiology, 10. Assay: Antioxidant in rat muscle tissue. Kumar, A., Singh, K. P., Bali, P., Anwar, S., Kaul, A., Singh, O. P. & Sinha, M. P. (2018). iNOS polymorphism modulates iNOS/NO expression via impaired antioxidant and ROS content in P. vivax and P. falciparum infection. Redox biology, 15, 192-206. Assay: Antioxidant in human serum. Xiao, R. (2018). Characterization and Cultivation of Marine Protist, Thraustochytrium striatum for High-Value Bioproducts. Assay: Antioxidant in mouse cells.Hernandez, O. E. A., Hernandez, L. H. H., Miyasaka, A. S., & Lugo, M. G. (2017). Effects of diets with whole plant-origin proteins added with different ratios of taurine: methionine on the growth, macrophage activity and antioxidant capacity of rainbow trout (Oncorhynchus mykiss) fingerlings. Veterinary and Animal Science, 3, 4-9. Assay: Antioxidant in fish liver tissue.Rishi, P., Arora, S., Kaur, U. J., Chopra, K., & Kaur, I. P. (2017). Better management of alcohol liver disease using a "Microstructured Synbox" System comprising L. plantarum and EGCG. PloS one, 12(1), e0168459. Assay: Antioxidant in rat liver tissue. Villa-Bellosta, R., Hamczyk, M. R., & Andres, V. (2017). Novel phosphate-activated macrophages prevent ectopic calcification by increasing extracellular ATP and pyrophosphate. PloS one, 12(3), e0174998. Assay: Antioxidant in mice marrow. Hasaninasab, A., Esteghamati, A., Azam, K., Yosaee, S., Habibi, N., & Djafarian, K. (2016). Comparison of serum Total Antioxidant Capacity between metabolic syndrome patients and non-metabolic syndrome people with normal or high BMI. Journal of Nutritional Sciences and Dietetics. Assay: Antioxidant in human serum.Subathra, T. A., Pal, G. K., Dhanalakshmi, Y., Nanda, N., & Swaminathan, R. P. (2016). Association of level of cognition with sympathovagal imbalance and cardiovascular risks in prehypertension. International Journal of Clinical and Experimental Physiology, 3(4), 197-203. Assay: Antioxidant in human blood.Hissa, MR et al (2015). A 16-week study to compare the effect of vildagliptin versus gliclazide on postprandial lipoprotein concentrations and oxidative stress in patients with type 2 diabetes inadequately controlled with metformin monotherapy. Diabetol Metab Syndr. 11;7:62. Assay: Antioxidant in human serum. Madrigal-Bujaidar, E et al (2015). Hepatoprotective effect of Geranium schiedeanum against ethanol toxicity during liver regeneration. World J Gastroenterology. 21(25): 7718-7729. Assay: Antioxidant in rat liver cells.Madrigal-Santillan, E et al (2015). Hepatoprotective effect of Geranium schiedeanum against ethanol toxicity during liver regeneration. World J Gastroenterology. 21(25): 7718-7729. Assay: Antioxidant in wistar rat liver cells. Hegazi, RM and Kamel HFM (2014). Oxidant Hepatic & /or Haem. Injury on Fuel-StationWorkers Exposed to Benzene Vapor, Possible Protectionof Antioxidants. American Journal of Medicine and Medical Sciences 4(2): 35-46. Assay: Antioxidant in human serum.Ijomone, OM, Olaibi, OK, Nwoha, PU, (2014). Effects of chronic nicotine administration on body weight, food intake and nitric oxide concentration in female and male rats. Pathophysiology. 21:185-190. Assay: Antioxidant in wistar rat serum. Olaibi, OK et al (2014). Histological and Histomorphometric studies of ethanol-injured pylorus and duodenum of Wistar rats pre-treated with Moringa oliefera extract. Al Am een J Med Sci. 7(2):104-111. Assay: Antioxidant in human duodenum tissue. Fawole, O., et al. (2011). Chemical and Phytochemical Properties and Antioxidant Activities of Three Pomegranate Cultivars Grown in South Africa. Food and Bioprocess Technology:1-7. Assay: Antioxidant in plant pomegranate juice.Hadzovi -Dzuvo, A et al (2011). Serum total antioxidant capacity in patients with multiple sclerosis. Bosnian Journal of Basic Medical Sciences 11(1):33-36. Assay: Antioxidant in human serum. Hadzovi -Dzuvo, A., et al. (2011). Serum total antioxidant capacity in patients with multiple sclerosis. Bosnian Journal of Basic Medical Sciences 11(1):33-36. Assay: Antioxidant in human serum.Hadzovic-Dzuvo, A et al (2011). Serum total antioxidant capacity in patients with multiple sclerosis. Bosnian Journal of Basic Medical Sciences 11(1):33-36. Assay: Antioxidant in human serum.Labib, HM, et al (2010). The Role of Oxidative Stress Markers and Nitric Oxide Levels in the Pathogenesis of Glaucoma. Austr. J. Basic and Applied Sci 4(8): 3553-3558. Assay: Total Oxidant in human blood. To find more recent publications, pleaseclick here.

If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.Simply send us your samples:- Fast turnaround - Quality data - Low costPlease email or call 1-510-782-9988 x 2 to request assay service.

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BioAssay Systems开发和销售创新的高通量分析解决方案，以满足生命科学行业不断增长的需求。BioAssay Systems的创始人在现代药物发现领域拥有50多年的丰富经验。在过去的15年中，我们已经开发了200多种用于药物发现和生命科学研究的检测试剂盒。我们以提供的服务和简单，易用且性能卓越的产品而感到自豪。

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