EnzyChrom™ Monoamine Oxidase Inhibitor Screening Kit

Application

• HTS for inhibitor screening and evaluation of monoamine oxidase inhibitors.

Key Features

• Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved.
• Robust and amenable to HTS: can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method

• FL530/585nm

Samples

• Monoamine Oxidase

Species

• All

Size

• 100 tests

Shelf Life

• 6 months

More Details

• MONOAMINE OXIDASES (MAO, EC 1.4.3.4) are a family of mitochondrial enzymes that catalyze the oxidative deamination of monoamines. Two isoforms of MAO exist, MAO-A and MAO-B, with different inhibitor selectivity and tissue distribution. MAO dysfunction is thought to be responsible for a number of neurological disorders. Unusually high or low levels of MAOs in the body have been associated with depression, schizophrenia, substance abuse, attention deficit disorder, migraines, and irregular sexual maturation. MAO inhibitors are one of the major classes of drug prescribed for the treatment of depression, Parkinson’s and Alzheimer"s diseases. BioAssay Systems MAO Inhibitor Screening Assay Kit provides a convenient fluorimetric means to screen for MAO enzyme inhibitors. In the assay, MAO reacts with p-tyramine, a substrate for both MAO-A and MAO-B, resulting in the formation of H₂O₂, which is determined by a fluorimetric method (λ_em/ex = 585/530 nm). The assay is simple, sensitive, stable and high-throughput adaptable.