How do I store the kit?
This kit is shipped at room temperature. Upon receiving, please keep the kit in the freezer (-20 to -10°C) for long term stability.What samples have you tested?
Serum samples were tested. Since DAO is copper dependent, EDTA should be avoided in sample preparation.What is the benefit of using an internal standard?
Serum and plasma samples greatly influence the HRP reaction. In order to measure an appropriate ?RFU for H2O2 in a serum or plasma matrix, an internal standard is required.Will the assay kit work in a 384 well plate, cuvette, etc?
Yes, the assay can be used in any standard black plate or cuvette. Simply adjust the total reaction volume to your container. For 384 well use 20-90 μL, for a cuvette use 1 mL (some narrow cuvettes can hold 200-500 μL).Can I store unused reagents for future use?
Yes, unused reagents can be stored according to the assay protocol. Repeated freeze/thaw cycles of reagents should be avoided. Working Reagents should be made fresh for each assay and used within 1 hour.I don’t have the correct wavelength filter, will the assay still work?
Typically if you are within ± 20 nanometers the assay will still work, but have slightly reduced sensitivity. Some assays have a narrower peak than others, call our tech support for a spectrum to verify.For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.