I don"t have the correct wavelength filter, will the assay still work?
Typically if you are within ± 20 nanometers the assay will still work, but have slightly reduced sensitivity. Some assays have a narrower peak than others, call our tech support for a spectrum to verify.What is the shelf life of this kit?
The shelf life is 6 months from the date you received the kit. The reagents are ready to use. No reagent reconstitution is needed.Does the assay kit work in particular cells?
Yes, this assay works in all mammalian culture cells. We have not tested this kit in other cells, such as bacteria, yeast cells etc.How do I normalize the results?
One method to normalize the results is to divide the glucose uptake activity against total number of cells or total cellular protein in the assay well. This would require a protein determination kit (e.g. BioAssay Systems" QCPR-500 or QFPR-200).Can I store unused reagents for future use?
Yes, unused reagents can be stored according to the assay protocol. Repeated freeze/thaw cycles of reagents should be avoided.Do I need to use a standard curve with each assay run?
Yes, it is highly recommended.For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.