EnzyChrom™ Glucose Assay Kit

What are the differences between the three EnzyChrom Glucose assay kits?

EBGL - best at detecting low levels of glucose (as low as 5 µM)EGL2 - best at measuring high levels of glucose using a low wavelength filter (340 nm)EGL3 - best at measuring high levels of glucose using a high wavelength filter (565 nm)

Which of the three EnzyChrom Glucose assay kits can be used with urine, saliva, or serum samples?

EBGL - Not recommended for urine, saliva, or serum samplesEGL2 - Compatible with urine and serum samples, not recommended for saliva samplesEGL3 - Recommended for urine, saliva, and serum samples*Sample blanks are to be used when testing urine samples with EGL2 or EGL3

How specific is this assay (EBGL)?

Glucose oxidase is fairly specific for D-glucose and does not react with fructose, galactose, or xylose. It does react with the following sugars: 2-deoxy-D-glucose (11% relative activity compared to D-glucose), 4-o-methyl-D-glucose (7%) and mannose (3%).

Can this kit be used to measure glucose concentrations in cells?

Glucose is converted into glucose-6-phosphate within the cell, which may not react with our assay. We have not tested it, but if the concentration of free glucose in the cell is within the detection range (1 to 30 μM for fluorimetric assays) of the assay it could still work.For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.

Jager, Marianne, et al (2018). Inducible knockdown of endothelial protein tyrosine phosphatase-1B promotes neointima formation in obese mice by enhancing endothelial senescence. Antioxidants & redox signaling 30.7: 927-944. Assay: Glucose in mice serum. Das, Champak, et al (2017). Multiplexed and fully automated detection of metabolic biomarkers using microdialysis probe. Sensors and Actuators B: Chemical 238: 633-640. Assay: Glucose in glucose. He L et al (2014). Potential biomarker of metformin action. J Endocrinol. JOE-14-0084. Assay: Glucose in mice white bloold cell. Klippel, S et al (2012). Methyljasmonate displays in vitro and in vivo activity against multiple myeloma cells. British Journal of Haematology, 159: 340-351.Assay: Glucose in cell line. Drew BG, et al (2011). Reconstituted high-density lipoprotein infusion modulates fatty acid metabolism in patients with type 2 diabetes mellitus. J Lipid Res. 52(3):572-81. Assay: Glucose in human plasma. Dhar A, et al (2010). Alagebrium attenuates acute methylglyoxal-induced glucose intolerance in Sprague-Dawley rats. Br J Pharmacol.159(1):166-75. Assay: Glucose in human plasma. Cha SH et al (2008). Differential effects of central fructose and glucose on hypothalamic malonyl-CoA and food intake. PNAS 105(44):16871-5. Assay: Glucose in mouse blood. Wolfgang MJ et al (2007). Regulation of hypothalamic malonyl-CoA by central glucose and leptin. PNAS 104(49):19285-90. Assay: Glucose in mouse blood. Wolfgang MJ et al (2006). The brain-specific carnitine palmitoyltransferase-1c regulates energy homeostasis. PNAS 103(19):7282-7. Assay: Glucose in mouse plasma.To find more recent publications, pleaseclick here.