EnzyChrom™ Oxalate Assay Kit

How do I store the kit?

This kit is shipped on ice. Upon receiving, please keep the kit in the freezer (-20 to -10°C).

What samples have you tested?

The assay kit has been tested with human serum, rat serum, human plasma, plant tissue samples, and human urine. Please follow the directions on the protocol for sample pretreatment.

How do I prepare cell or tissue samples for assays?

Follow the general protocol below for cell and tissue samples.Cell Samples. Suspend about two million (2 × 10⁶) harvested cells in 400 µL PBS on ice. Lysis can be achieved by homogenization (10-20 passes in a Dounce homogenizer on ice) or by sonication (preferably performed in a ice-water bath). The degree of cell lysis can be checked under a microscope. Centrifuge homogenate at 14,000 g for 10 min. Transfer the clear supernatant into a clean tube. It is prudent to run a pilot test of the sample at different dilutions. Choose a dilution with the readings in the linear range of the standard curve for further assays. Most samples can be stored at -80°C if not assayed immediately.Tissue Samples. Cut the tissue into small pieces and weigh 20-100 mg tissue, then add 200- 1000 µL ice-cold PBS. Lysis can be achieved by homogenization (10-20 passes in a Dounce homogenizer on ice) or by sonication (preferably performed in an ice water bath). The degree of tissue lysis can be checked under a microscope. Centrifuge homogenate at 14,000g for 10 min. Transfer the clear supernatant into a clean tube. It is prudent to run a pilot test of the sample at different dilutions. Choose a dilution with the readings in the detection range of the standard curve for further assays. Most samples can be stored at -80°C if not assayed immediately.

What is the benefit of using an internal standard?

Some assays encounter interference and the values cannot be compared to a standard curve. The use of an internal standard corrects for potential interference.

I don’t have the correct wavelength filter, what other wavelength(s) would work?

The assay can be measured at 550 - 600 nm with 595 nm as the peak. Reading outside this range will result in a significant decrease in sensitivity.

Can I store unused reagents for future use?

Yes, unused reagents can be stored according to the assay protocol. Repeated freeze/thaw cycles of reagents should be avoided. Working Reagents should be made fresh for each assay and used within 2 hours.

Do I need to use a standard or standard curve with each assay run?

Yes, it is highly recommended.For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.

Fukano, Y. (2017). Vine tendrils use contact chemoreception to avoid conspecific leaves. Proceedings of the Royal Society B: Biological Sciences, 284(1850), 20162650. Assay: Oxalate in leaf tissue.To find more recent publications, pleaseclick here.