QuantiChrom™ Acetylcholinesterase Assay Kit

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ProtocolSDS

Application

For quantitative determination of acetylcholinesterase activity and evaluation of acetylcholinesterase inhibitors.

Key Features

Sensitive and accurate. Detection range 10 to 600 U/L AChE activity in 96-well plate assay.
Convenient. The procedure involves adding a single working reagent, and reading the optical density at 2 min and 10 min at room temperature.
High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

Method

OD412nm

Samples

Blood, serum, plasma etc

Species

Size

100 tests

Detection Limit

10 U/L

Shelf Life

6 months

More Details

ACETYLCHOLINESTERASE (EC 3.1.1.7, AChE), also known as RBC cholinesterase, is found primarily in the blood and neural synapses. Low serum cholinesterase activity may relate to exposure to insecticides or to one of a number of variant genotypes. AChE catalyzes the hydrolysis of the neurotransmitter acetylcholine into choline and acetic acid, a reaction necessary to allow a cholinergic neuron to return to its resting state after activation. Cholinesterase levels of cells and plasma are used as a guide in establishing safety precautions relative to exposure and contact, as well as a guide in determining the need for workers to be removed from areas of contact with the organic phosphate insecticides. Simple, direct and automation-ready procedures for measuring AChE activity are very desirable. BioAssay Systems QuantiChrom™ Acetylcholinesterase Assay is based on an improved Ellman method,in which thiocholine produced by the action of acetylcholinesterase forms a yellow color with 5,5’-dithiobis(2-nitrobenzoic acid). The intensity of the product color, measured at 412 nm, is proportionate to the enzyme activity in the sample.

Does the assay detect only acetylcholinesterase activty?

No, this assay detects acetylcholinesterase and butyrylcholinesterase (also known as plasma cholinesterase, pseudocholinesterase) and does not distinguish between the two.

Which anticoagulant should I use for blood sample collection?

We recommend using serum for measuring plasma cholinesterase activity and EDTA blood for measuring red blood cell acetylcholinesterase activity.For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.

Aydin B, Cabadak H, Goren MZ (2018). Investigation of the Roles of Non-neuronal Acetylcholine in Chronic Myeloid Leukemic Cells and their Erythroid or Megakaryocytic Differentiated Lines. Anticancer Agents Med Chem. 18(10):1440-1447. Assay: Acetylcholinesterase in human K562 cells. Margus, A. (2018). Adaptation to stressful environments: invasion success of the Colorado potato beetle (Leptinotarsa decemlineata). JYU dissertations, (11). Assay: Acetylcholinesterase in beedle larvae.McCloskey, M et al (2017). The influence of acetylcholinesterase on anxiety- and depression-like behaviors in fluoxetine-treated male mice. BIOS 88(1), 29-38. Assay: Acetylcholinesterase in mice brain cells.Musa, N. H., Mani, V., Lim, S. M., Vidyadaran, S., Majeed, A. B. A., & Ramasamy, K. (2017). Lactobacilli-fermented cow"s milk attenuated lipopolysaccharide-induced neuroinflammation and memory impairment in vitro and in vivo. Journal of Dairy Research, 84(4), 488-495. Assay: Acetylcholinesterase in mice brain cells. Sorial, M. E., & El Sayed, N. S. E. D. (2017). Protective effect of valproic acid in streptozotocin-induced sporadic Alzheimer"s disease mouse model: possible involvement of the cholinergic system. Naunyn-Schmiedeberg"s archives of pharmacology, 390(6), 581-593. Assay: Acetylcholinesterase in mice. Ahmad, MZ et al (2015). Impact of chlorpyrifos on health biomarkers of broiler chicks. Pestic Biochem Physiol. 122:50-8. Assay: Acetylcholinesterase in broiler birds serum. Mineur YS, et al. (2013) Cholinergic signaling in the hippocampus regulates social stress resilience and anxiety- and depression-like behavior. Proc Natl Acad Sci U S A 110: 3573-3578. Assay: Acetylcholinesterase in mouse brain nuclei. Khokhar JY and Tyndale RF (2012). rat brain CYP2B-enzymatic activation of chlorpyrifos to the oxon mediates cholinergic neurotoxicity. Toxicol Sci. 126:325-35. Assay: Acetylcholinesterase in rat serum. Gupta, A et al (2011). Peripheral insulin-sensitizer drug metformin ameliorates neuronal insulin resistance and Alzheimer"s-like changes. Neuropharmacology 60(6):910-20. Assay: Acetylcholinesterase in neuronal cell line. Ng J, et al (2011). Autonomic remodeling in the left atrium and pulmonary veins in heart failure: creation of a dynamic substrate for atrial fibrillation. Circ Arrhythm Electrophysiol.4(3):388-96. Assay: Acetylcholinesterase in dog explanted atria. Al-Akwa AA, et al (2009). Free radicals are present in human serum of Catha edulis Forsk (Khat) abusers. J Ethnopharmacol. 125(3):471-3. Assay: Acetylcholinesterase in human serum. Basselin M, et al (2009). Acute but not chronic donepezil increases muscarinic receptor-mediated signaling via arachidonic acid in unanesthetized rats. J Alzheimers Dis. 17(2):369-82. Assay: Acetylcholinesterase in rat cerebral cortical tissues.To find more recent publications, pleaseclick here.

If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.Simply send us your samples:- Fast turnaround - Quality data - Low costPlease email or call 1-510-782-9988 x 2 to request assay service.

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